The viable cells were then counted using the trypan blue exclusion technique. The cultures were exposed for 1, 2, 4, and 8 h to experimental solutions (tap water served as negative control and DMEM as positive control) at 37Â☌.
PDL cells were obtained from extracted healthy third molars and cultured in Dulbecco's modified Eagles medium (DMEM). The purpose of this study was to evaluate the ability of soymilk, powdered milk, and Hank's balanced salt solution (HBSS) to maintain human periodontal ligament (PDL) cell viability in vitro. Moazami, Fariborz Mirhadi, Hosein Geramizadeh, Bita Sahebi, Safoura
PMID:22413949Ĭomparison of soymilk, powdered milk, Hank's balanced salt solution and tap water on periodontal ligament cell survival. Further investigations are needed to examine the degradation behavior. Conclusions The results of the present in vitro study indicate that ZEK100 is a potential candidate for degradable orthopedic implants. This indicates the precipitation of calcium phosphates with low solubility on the surface of the ZEK100 plates. The characterization of the surface revealed the presence of high amounts of O, P and Ca on the surface and small Mg content. Results The four point bending test displayed an increased bending strength after 6 weeks immersion compared to the 2 week group and 4 week group. The surfaces of the immersed specimens were characterized by SEM, EDX and XRD. After 14, 28 and 42 days of immersion the ZEK100 plates were mechanically tested via four point bending test. Methods ZEK100 plates were examined in this in vitro study with Hank's Balanced Salt Solution under physiological conditions with a constant laminar flow rate. Particularly in orthopedic research magnesium is interesting because of its mechanical properties close to those of natural bone, the prevention of both stress shielding and removal of the implant after surgery. In vitro corrosion of ZEK100 plates in Hank's Balanced Salt Solutionīackground In recent years magnesium alloys have been intensively investigated as potential resorbable materials with appropriate mechanical and corrosion properties.